gene-cloning-and-construction-of-expression-vector

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Gene Transfer And Expression In Mammalian Cells

Author : S.C. Makrides
ISBN : 9780080930350
Genre : Science
File Size : 20. 35 MB
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The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors. This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field.

Cumulated Index Medicus

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ISBN : UIUC:30112041289528
Genre : Medicine
File Size : 28. 67 MB
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Bibliography Of Agriculture

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ISBN : PSU:000057553993
Genre : Agriculture
File Size : 47. 74 MB
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Cloning And Expression Vectors For Gene Function Analysis

Author : Quinn Lu
ISBN : CORNELL:31924086745670
Genre : Science
File Size : 42. 57 MB
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Index Medicus

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ISBN : OSU:32436011091582
Genre : Medicine
File Size : 48. 62 MB
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Genetics Abstracts

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ISBN : UOM:39015042127988
Genre : Genetics
File Size : 63. 64 MB
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Biomedical Index To Phs Supported Research

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ISBN : STANFORD:36105119624620
Genre : Medicine
File Size : 34. 77 MB
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Recombinant Dna Principles And Methodologies

Author : James Greene
ISBN : 0824799895
Genre : Science
File Size : 29. 5 MB
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This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

Biotechnology Research Abstracts

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ISBN : UCLA:L0067363093
Genre : Biochemical engineering
File Size : 22. 41 MB
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Monthly. Classified listing of references to worldwide articles dealing with all aspects of biotechnology. Also includes books and conferences. Each entry gives bibliographic information, institutional address of author(s), and abstract. Author and subject index.

Cambridge Scientific Biochemistry Abstracts

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ISBN : UVA:X001853240
Genre : Nucleic acids
File Size : 43. 4 MB
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Engineering And Construction Of Expression Systems For The Production Of Recombinant Pharmaceutical Proteins

Author : Christopher Carl Frye
ISBN : IND:30000082018221
Genre :
File Size : 28. 91 MB
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Construction And Characterization Of The Bacteriophage Lambda Charon Vectors For Dna Cloning

Author : Bill Gary Williams
ISBN : WISC:89010839728
Genre : Molecular cloning
File Size : 75. 94 MB
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Recombinant Dna Research And Viruses

Author : Yechiel Becker
ISBN : 0898386837
Genre : Medical
File Size : 85. 5 MB
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The development of recombinant DNA technology has made a marked impact on molecular virology. The cleavage of viral DNA genomes with restriction enzymes and the cloning of such DNA fragments in bacterial p1asmids has led to the amplification of selected viral DNA fragments for sequencing and gene expression. RNA virus genomes which can be transcribed to their cDNA form were also cloned in bacterial p1asmids, facilitating the study of RNA virus genes. With the elucidation in recent years of the promoter sequence of various viral genes and the expression of these genes in bacteria or yeast, the understanding of many viral gene functions has made great progress. Cloning and expression of viral genes in mammalian cells was made possible by the construction of shuttle plasmid vectors which carry the origins of DNA replication from bacteria and/or mammalian viruses. The expression of viral genes in bacteria, yeast and eukaryotic cells gives reason to hope that it will be possible to produce viral antigens in large quantities for use as human or animal vaccines. The present volume attempts to capture for the reader some of the high lights of recombinant DNA research in the field of animal and plant viruses.

Nucleic Acids Abstracts

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ISBN : UVA:X006074556
Genre : Nucleic acids
File Size : 89. 67 MB
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Gene Cloning

Author : T. A. Brown
ISBN : STANFORD:36105032991262
Genre : Biological sciences
File Size : 68. 41 MB
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Third edition. This concise and accessible introduction to gene cloning assumes almost no background knowledge.

Recombinant Dna Technology

Author : Keya Chaudhuri
ISBN : 9788179933206
Genre : Science
File Size : 81. 86 MB
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Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences.

The Construction And Expression Of Recombinant Anti Acetylcholine Receptor Monoclonal Antibodies To Study The Role Of Complement Activation In Experimental Autoimmune Myasthenia Gravis

Author : Christine Ann Kirvan
ISBN : UCAL:X61280
Genre :
File Size : 82. 82 MB
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Preparation Of An Infectious Molecular Clone And Analysis Of The Vif Gene Of A Feline Immunodeficiency Virus Fiv Oma

Author : Fan Long
ISBN : CORNELL:31924076520455
Genre :
File Size : 83. 22 MB
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Biochemistry Abstracts

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ISBN : UCAL:B3873720
Genre : Biochemistry
File Size : 34. 68 MB
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Virology Abstracts

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ISBN : PSU:000056035094
Genre : Virology
File Size : 59. 52 MB
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