cell-penetrating-peptides

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Cpp Cell Penetrating Peptides

Author : Ülo Langel
ISBN : 9789811387470
Genre : Science
File Size : 26. 96 MB
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In this book, a summary and update of the most important areas of CPP research are presented, whilst raising relevant questions for further development. The CPP sequences are presented and discussed throughout the book. The methods for testing CPP mechanisms are discussed in detail. Various approaches for the testing of endocytotic pathways of CPP uptake are also described. Different CPP uptake experiments are compared since it is becoming clear that it is often best to apply several methods in a complementary manner in order to most comprehensively evaluate CPP uptake mechanisms due to the complexity of these processes. A brief summary of functionality issues of CPPs, both in vitro and in vivo are discussed. Therapeutic potential of CPPs and commercial developments are discussed. The monograph is written for researchers and students in the field.

Cell Penetrating Peptides

Author : Ulo Langel
ISBN : 9781420040777
Genre : Medical
File Size : 36. 84 MB
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One of the major problems with drug delivery is the inability of large hydrophilic compounds to pass through the lipid membrane of the cell, thus making drugs such as polypeptides and oligonucleotides of limited therapeutic value. Until recently, the transport of such molecules into the cytoplasmic and nuclear compartments of living cells seemed a

Handbook Of Cell Penetrating Peptides

Author : Ulo Langel
ISBN : 1420006088
Genre : Medical
File Size : 71. 16 MB
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Since the first Handbook of Cell-Penetrating Peptides was prepared in 2001, the wealth of new information on the use of these peptides as transport systems has in fact served to confound the field. The constant internal change in the field of cell-penetrating peptides (CPPs) is due to recent research uncovering apparent ambiguities in cellular upta

Cell Penetrating Peptides

Author : Ülo Langel
ISBN : 1493949624
Genre : Science
File Size : 73. 8 MB
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Divided into three parts this volume summarizes the most important areas of Cell-Penetrating Peptides (CPP) research . Part one briefly presents the historical background of CPP studies and the classifications of the available CPPs, and then summarizes the approaches for prediction of novel CPPs. Part two mainly describes the methods for studies of “naked” CPPs, that is, CPPs without conjugated cargos. Last but not least part three presents a representative and brief summary of functionality issues of CPPs, both in vitro and in vivo. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Cell-Penetrating Peptides: Methods and Protocols, Second Edition hopes to raise relevant questions for further development.

Characterization Of Cell Penetrating Peptide Complexation And Interaction With Plant Cells

Author : Jordan Tan Pepper
ISBN : OCLC:1227985944
Genre :
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Cell penetrating peptides (CPPs) are short 8-30 amino acid polypeptides that are able to transit across the semi-permeable plasma membrane of living cells, and deliver bound cargoes to the cytoplasm and other subcellular locations (e.g. nucleus, plastid organelles etc). The use of CPPs in plant cells and tissues remains a small and little researched field compared with the greater body of animal based research imperatives. The present research describes firstly, the measured interaction of the chosen model CPP Tat¬2 with the cell wall of microspores (i.e. microspore exine), secondly the reduction of the size and increase in regularity (measured as polydispersity) of Tat2 nucleic acid polyplexes and the unique empirical derivation of pseudo-kinetic parameters of nucleic acid CPP aggregation. Additionally several attempts to apply the findings from this research in plant cells and tissues are presented and discussed throughout.

Characterization And Evaluation Of Hybrid Collagen Cell Penetrating Peptides For Sirna Delivery

Author : Alicia Gamboa
ISBN : 043820901X
Genre : Peptides
File Size : 25. 94 MB
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Abstract: Short interfering RNA (siRNA) causes sequence specific gene silencing of mRNA and has been shown to be a very promising therapeutic agent for a wide range of diseases. We have developed a hybrid collagen/cell penetrating peptide (CHP), that contains a triple helical domain (POG)n that provides stability, and a cell penetrating domain (Rn) which contains positively charged arginine residues allowing for internalization into cells. We determined that the CHPs form highly crystalline nanoparticles with siRNA with molar ratios of 1:18 and 1:9 depending on the number of arginines in the CPP domain. CHPs are able to effectively deliver and release siRNA into 3T3 Swiss mouse fibroblast cells with higher efficiency and gene silencing ability than that of Lipofectamine. The data suggest that our strategy for development of the CHP-siRNA complex can provide an avenue for effective delivery of siRNA.

Molecular And Structural Aspects Of Cell Penetrating Peptides Membrane Crossing

Author : Chérine Bechara
ISBN : OCLC:1225626592
Genre :
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Les peptides vecteurs (ou cell-penetrating peptides CPPs) ont émergé comme une nouvelle classe de transporteurs vers le milieu intracellulaire de macromolécules d'intérêt. Néanmoins, et afin de mieux utiliser ces vecteurs, il faut en comprendre les mécanismes d'entrée ainsi que leur localisation finale dans les cellules. Dans ce contexte, mon travail vise à mieux comprendre les interactions moléculaires entre les CPPs basiques et les constituants de la membrane plasmique, qui pourront être impliqués dans l'internalisation de ces CPPs. Les différents résultats montrent qu'une meilleure affinité à la membrane plasmique d'un peptide ne corrèle pas avec une meilleure efficacité d'internalisation. Par ailleurs, les glycosaminoglycanes (GAGs) sont démontrés comme étant des acteurs important de l'endocytose des CPPs par agrégation des complexes CPPs/GAGs. La formation d'agrégats stables n'a été observée que pour les CPPs basiques qui comportent des résidus tryptophane dans leur séquence, qui se structurent en brin b suite à la complexation avec les GAGs. En absence de résidus tryptophane, un nombre critique de résidus arginine confère cependant aux séquences peptidiques des capacités d'internalisation similaires à celles des peptides avec tryptophane. Par ailleurs, l'hydrolyse de la sphingomyéline et la formation de domaines riches en céramide augmente l'entrée des CPPs dans les cellules sauvages plus que dans les cellules déficientes en GAGs. De plus, l'augmentation de l'entrée est plus marquée pour les peptides comprenant des résidus tryptophane, démontrant que la formation des domaines riches en céramide augmente l'endocytose des CPPs déclenchée par l'agrégation des GAGs.

Optimization Of Cell Penetrating Peptide Based Microrna 146a Delivery Into Dendritic Cells For Targeting Of Skin Inflammation

Author : Gemma Carreras Badosa
ISBN : OCLC:1163843885
Genre :
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MiRNAs are gene expression regulators with potential therapeutic applications. We have previously shown that miR-146a mimics and cell penetrating peptide (CPP) PepFect6 form noncovalent nanocomplexes, which suppress inflammation keratinocytes (KCs) and in a mouse model of irritant contact dermatitis. As in the skin, KCs, Langerhanu2019s cells and dermal dendritic cells (DCs) are main cell types exposed to therapeutic targeting, we here aimed to test the capacity of selected CPPs in delivery of miRNA mimics into KCs and in vitro differentiated DCs. We used PepFect (PF) and NickFect (NF) type of CPPs and show that they both support delivery of fluorescently labelled miRNA mimics into KCs and to a lesser extent to DCs. In KCs, the downregulation of miR-146a-influenced genes was evident with both PF and NF type peptides, while in DCs, only NF-delivered miR-146a was efficient in the suppression of the target genes. Any of the tested CPPs did not cause immune activation of DCs. Transmission electron microscopy analysis with nanogold labelled miR-146a mimics showed that in case of NFs, endocytosis is an active route of delivery in KCs and DCs. While in NF-miR-146a complexes were found mainly in endosomes and cytoplasm, the PF-miR-146a nanocomplexes were detected more often at the surface of DCs, indicating that they either enter poorly or are subjected to antigen presenting. In conclusion, NFs efficiently transport miRNA mimics into KCs and DCs and therefore may have advantage in therapeutic delivery of miRNAs. Further studies will be needed to test the efficiency of NF-miR146a nanocomplexes in skin inflammation in vivo.

Official Gazette Of The United States Patent And Trademark Office

Author :
ISBN : WISC:89085755585
Genre : Trademarks
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The Future Of Pharmaceutical Product Development And Research

Author :
ISBN : 9780128144558
Genre : Medical
File Size : 79. 34 MB
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The Future of Pharmaceutical Product Development and Research examines the latest developments in the pharmaceutical sciences, also highlighting key developments, research and future opportunities. Written by experts in the field, this volume in the Advances in Pharmaceutical Product Development and Research series deepens our understanding of the product development phase of drug discovery and drug development. Each chapter covers fundamental principles, advanced methodologies and technologies employed by pharmaceutical scientists, researchers and the pharmaceutical industry. The book focuses on excipients, radiopharmaceuticals, and how manufacturing should be conducted in an environment that follows Good Manufacturing Practice (GMP) guidelines. Researchers and students will find this book to be a comprehensive resource for those working in, and studying, pharmaceuticals, cosmetics, biotechnology, foods and related industries. Provides an overview of practical information for clinical trials Outlines how to ensure an environment that follows Good Manufacturing Practice (GMP) Examines recent developments and suggests future directions for drug production methods and techniques

Cell Penetrating Versions Of Caspase Targeting Activity Based Probes

Author : Sheng Zhi Chia
ISBN : STANFORD:36105128087470
Genre :
File Size : 20. 62 MB
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An Investigation Of Erbb1 Signalling Pathways Using Cell Penetrating Peptides

Author : L. Devine
ISBN : OCLC:1064869641
Genre :
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Design Of Cancer Selective And Nuclei Targeting Cell Penetrating Peptides And Their Characterization

Author : Anja Gronewold
ISBN : OCLC:1028683649
Genre :
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Design Strategy Of Cell Penetrating Copolymers For High Efficient Drugdelivery

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ISBN : OCLC:1051933775
Genre :
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Abstract: Finding a highly effective drug delivery carrier with low cytotoxicity is essential for disease therapy. In this work, we design a cell-penetrating copolymer (CPC) carrier, in which the inspiration comes from cell-penetrating peptides that have both hydrophilic and hydrophobic residues and are capable of penetrating membranes without inducing membrane disruption. Further dissipative particle dynamics simulations indicate that the CPCs also have an effective penetration capacity. Importantly, we found that the penetration mechanism of the CPC is in a zipper way, i.e. the adjacent hydrophobic segments of the CPC could cross the membrane in a cooperative way. Moreover, we determine the optimal parameters for the CPC crossing lipid membrane, i.e. the hydrophobic segment length of the CPC is close to the membrane thickness, and the CPC has more segment number. Finally, by grafting the CPC with the optimal structure on the hydrophilic drug, we found that the CPCs can definitely help the hydrophilic drug penetrate the lipid membrane effectively, which is an excellent prototype of drug delivery carriers. It is expected that this work can provide the fundamental for further design of drug delivery carriers.

Development Of Peptides As Potential Antivirals Against Herpes Simplex Virus Type 1 Infection

Author : Radeekorn Akkarawongsa
ISBN : WISC:89098969645
Genre :
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Handbook Of Pharmaceutical Biotechnology

Author : Shayne C. Gad
ISBN : UOM:39015064951935
Genre : Medical
File Size : 64. 21 MB
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Describes the use of biotechnology to develop pharmaceuticals. This book gives the professional a basic tool to facilitate the development of biotech medicines by bringing together a general overview of biotechnology used in the drug development process, along with a compendium of regulations and validation methods.

The Journal Of Cell Biology

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ISBN : UOM:39015072632964
Genre : Cytology
File Size : 36. 30 MB
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No. 2, pt. 2 of November issue each year from v. 19 (1963)-47 (1970) and v. 55 (1972)- contain the Abstracts of papers presented at the Annual Meeting of the American Society for Cell Biology, 3d (1963)-10th (1970) and 12th (1972)-

Innovation And Perspectives In Solid Phase Synthesis Combinatorial Libraries

Author : Roger Epton
ISBN : STANFORD:36105126892152
Genre : Nucleic acids
File Size : 83. 29 MB
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Drug Tranport Ers And The Diseased Brain

Author : Fundación Dr. Antonio Esteve. Symposium
ISBN : UOM:39015062462075
Genre : Medical
File Size : 53. 45 MB
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There are many potent drugs available for many diseases of the brain (e.g. Alzheimer's Disease, Parkinson's Disease, Multiple Sclerosis, Stroke, Brain tumors) The effectiviness however is not optimal since barriers in the brain prevent the drugs from reaching the brain in sufficient therapeutic concentrations (e.g. the blood-brain barrier and the blood-cerebrospinal fluid barrier). Drug Transport(ers) and the Diseased Brain contains the papers of a timely symposium held in Spain between 6 and 9 October 2004, which sets out not only the point of view of the academic researcher and the clinician, but also from the pharmaceutical industrial on how to overcome these barriers and how to treat brain diseases effectively. Blood-brain barrier and blood-cerebrospinal fluid-barrier drug transport; Drug targeting to the brain; Drug transporters at barriers in the brain

Canadian Journal Of Physiology And Pharmacology

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ISBN : UCSD:31822036145985
Genre : Pharmacology
File Size : 85. 53 MB
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